Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECALPS) Occurs in Rough Strains of Escherichia coli R1, R2, and R4
نویسندگان
چکیده
منابع مشابه
Immunogenicity of the common enterobacterial antigen produced by smooth and rough strains.
Enterobacteriaceae share a common antigen (CA). This antigen exists as a powerful immunogen, when produced by Escherichia coli O14, and as a minimally effective immunogen, when present in cultures of most other smooth strains. The present study was directed toward the assessment of immunogenicity of this antigen produced by various rough mutants and their parent strains as well as that of viabl...
متن کاملIdentification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli.
Phosphoglyceride-linked enterobacterial common antigen (ECA(PG)) is a cell surface glycolipid that is synthesized by all gram-negative enteric bacteria. The carbohydrate portion of ECA(PG) consists of linear heteropolysaccharide chains comprised of the trisaccharide repeat unit Fuc4NAc-ManNAcA-GlcNAc, where Fuc4NAc is 4-acetamido-4,6-dideoxy-D-galactose, ManNAcA is N-acetyl-D-mannosaminuronic a...
متن کاملBiosynthesis of Enterobacterial Common Antigen in Esc~erichia coli
Twelve independent TnlO insertion mutants of Escherichia coli K12 were isolated that were defective in the synthesis of enterobacterial common antigen (ECA). The mutants were identified by screening a random pool of TnlO insertion mutants for their ECA phenotype using a colony-immunoblot assay. All 12 of the TnlO insertion mutants were found to be located in the chromosomal region of the rff-rf...
متن کاملAssembly of cyclic enterobacterial common antigen in Escherichia coli K-12.
We describe here the purification and quantification of a water-soluble cyclic form of enterobacterial common antigen (ECA(CYC)) from Escherichia coli K-12 as well as information regarding its subcellular location and the genetic loci involved in its assembly. Structural characterization of purified ECA(CYC) molecules obtained from E. coli K-12 revealed that they uniformly contained four trisac...
متن کاملEnterobacterial common antigen.
INTRODUCTION .............. ................................ 591 DISCOVERY AND DEFINITIONS ............................................. 591 SEROLOGICAL METHODS ............................................. 592 Hemagglutination (HA), Hemolysis, and Hemagglutination Inhibition (HAI) ... 592 Other Serological Methods ............................................. 593 CHEMISTRY .......................
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2020
ISSN: 1422-0067
DOI: 10.3390/ijms21176038